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6/11/2012, · The main methods used for ,HPV, integration ,detection, are ,PCR,, ,fluorescence, in situ hybridization, and Real-Time ,PCR,. The latter allows calculation of the ratio between the levels of E2 and E6/E7 ,HPV, genes. When there is ,HPV, integration, the viral genome shows a …
1/5/1998, · In SiHa cells, containing 1–2 copies of HPV DNA type 16, we attempted to detect HPV DNA by various methods such as enzymatic in situ hybridization (EISH) and in situ PCR combined with observations with brightfield microscopy, and fluorescent in situ hybridization (FISH) associated with observations with LSCM.
FDA-approved commercially available method for the detection of HPV DNA is the Hybrid Capture assay, version hc2 (Digene, Gaithersburg, MD, USA) which is able to detect 13 high-risk types of HPV. The advantage of PCR-based methods of HPV DNA detection is that they allow for the identification of different types of HPV. This
portable ﬂuorescence reader. Up to 13 human papillomavirus types could be detected simultaneously by a single-step operation in less than 30 min after linear-after-the-exponential (LATE)-PCR. The sensitivity was determined to be 10−102 copies plasmid DNA/μL. The speciﬁcity study showed no cross-reactivity among the 31 diﬀerent common HPV types.
LineGene1600 Series ,Fluorescent, Quantitative ,PCR Detection, system Important Notes Important Notes 1. Usual practice Note: Very important information is contained within this manual and it should be carefully read before first use of the instrument.
A method for the ,detection, and quantitation of oncogenic ,human papillomavirus, (,HPV,) was developed by using the ﬂuorescent 5* exonuclease assay. The method is based on the ampliﬁcation of a 180-bp fragment from the 3* part of the E1 open reading frame in a single ,PCR, with type-speciﬁc probes for ,HPV, types 16, 18, 31, 33, and 35.
6/11/2012, · The main methods used for HPV integration detection are PCR, fluorescence in situ hybridization, and Real-Time PCR. The latter allows calculation of the ratio between the levels of E2 and E6/E7 HPV genes. When there is HPV integration, the viral genome shows a …
Objective To explore different application of ,PCR,-,fluorescence, method and ,PCR,-hybridization method in the diagnosis ,of human papilloma virus,(,HPV,)infection.Methods Clinical samples were collected and detected by ,PCR,-,fluorescence, method and ,PCR,-hybridization method respectively.The cell histological diagnosis was used to compare the positive rate of HPVDNA ,detection, between the two methods,and ...