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It has been confirmed that detection of high-risk human papillomavirus (HR HPV) DNA is useful in cervical cancer (CC) screening. Recently, a new real-time fluorescent polymerase chain reaction (PCR) assay was developed to detect HR HPV. This assay can synchronize nucleic acid amplifica-tion and testing using specific primers for 13 types of HR HPV
(2) The ,fluorescence, signal is obtained by embedding ,fluorescent, dye into double stranded DNA, or by combining ,fluorescent, probe with wood extract, which improves the sensitivity, specificity and accuracy of ,detection,. Real time o-,pcr, can be applied to the study of mRNA expression, the ,detection, of DNA copy number, the ,detection, of single ...
HPV, typing by ,PCR, specific for the ,HPV,-6, -11, -16, -18, -31 and -33 revealed that all GP5/6 ,PCR, (n = 12) contained ,HPV, types different from these 6 types. These data indicate that the GP5+/6+ ,PCR, method provides an increased ,detection, level mainly of uncommon, apparently poorly matched ,HPV, types in cervical scrapes and most likely in the enlargement of the spectrum of HPVs detectable by this ...
RESEARCH ARTICLE Open Access ,Detection of HPV, in oral rinse samples from OPSCC and non-OPSCC patients Juliet Dang1*, Qinghua Feng5, Keith D. Eaton2, Hona Jang5 and Nancy B. Kiviat3,4 Abstract Background: Due to the increasing rates of oropharyngeal cancer, oral ,HPV, …
The present invention relates generally to PCR-based assays to detect the presence of human papillomavirus (HPV) types in clinical samples. More specifically, it relates to fluorescent multiplex PCR assays, wherein multiple fluorophores are used to simultaneously detect a plurality of HPV loci in a single PCR reaction tube.
Objective To explore different application of ,PCR,-,fluorescence, method and ,PCR,-hybridization method in the diagnosis ,of human papilloma virus,(,HPV,)infection.Methods Clinical samples were collected and detected by ,PCR,-,fluorescence, method and ,PCR,-hybridization method respectively.The cell histological diagnosis was used to compare the positive rate of HPVDNA ,detection, between the two methods,and ...
Reliable, sensitive, and accurate detection of specific HPV types is critical for the study of cervical cancer causation as well as the support of vaccine clinical trials. Several PCR-based methodologies have been developed to amplify and detect HPV DNA ( 1, 4, 7, 8, 9, 12, 13 ).
A method for the detection and quantitation of oncogenic human papillomavirus (HPV) was developed by using the ﬂuorescent 5* exonuclease assay. The method is based on the ampliﬁcation of a 180-bp fragment from the 3* part of the E1 open reading frame in a single PCR with type-speciﬁc probes for HPV types 16, 18, 31, 33, and 35.
LineGene1600 Series ,Fluorescent, Quantitative ,PCR Detection, system Important Notes Important Notes 1. Usual practice Note: Very important information is contained within this manual and it should be carefully read before first use of the instrument.