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(2) The ,fluorescence, signal is obtained by embedding ,fluorescent, dye into double stranded DNA, or by combining ,fluorescent, probe with wood extract, which improves the sensitivity, specificity and accuracy of ,detection,. Real time o-,pcr, can be applied to the study of mRNA expression, the ,detection, of DNA copy number, the ,detection, of single ...
Real-time PCR is a highly sensitive target amplification technique available for HPV-DNA detection. Real-time PCR combines fluorescent probes with PCR primers, allowing for accurate quantification of virus present in a sample.
The increased sensitivity of fluorescence ISH (FISH) methods, allowing the detection of single copies of HPV, complicates the distinction between integrated and episomal HPV. Recently it has been suggested that, in such assays, the signals originating from integrated virus can …
Objective To explore different application of ,PCR,-,fluorescence, method and ,PCR,-hybridization method in the diagnosis ,of human papilloma virus,(,HPV,)infection.Methods Clinical samples were collected and detected by ,PCR,-,fluorescence, method and ,PCR,-hybridization method respectively.The cell histological diagnosis was used to compare the positive rate of HPVDNA ,detection, between the two methods,and ...
It has been confirmed that detection of high-risk human papillomavirus (HR HPV) DNA is useful in cervical cancer (CC) screening. Recently, a new real-time fluorescent polymerase chain reaction (PCR) assay was developed to detect HR HPV. This assay can synchronize nucleic acid amplifica-tion and testing using specific primers for 13 types of HR HPV
LineGene1600 Series ,Fluorescent, Quantitative ,PCR Detection, system Important Notes Important Notes 1. Usual practice Note: Very important information is contained within this manual and it should be carefully read before first use of the instrument.
A method for the detection and quantitation of oncogenic human papillomavirus (HPV) was developed by using the ﬂuorescent 5* exonuclease assay. The method is based on the ampliﬁcation of a 180-bp fragment from the 3* part of the E1 open reading frame in a single PCR with type-speciﬁc probes for HPV types 16, 18, 31, 33, and 35.
The present invention relates generally to PCR-based assays to detect the presence of human papillomavirus (HPV) types in clinical samples. More specifically, it relates to fluorescent multiplex PCR assays, wherein multiple fluorophores are used to simultaneously detect a plurality of HPV loci in a single PCR reaction tube.
HPV, typing by ,PCR, specific for the ,HPV,-6, -11, -16, -18, -31 and -33 revealed that all GP5/6 ,PCR, (n = 12) contained ,HPV, types different from these 6 types. These data indicate that the GP5+/6+ ,PCR, method provides an increased ,detection, level mainly of uncommon, apparently poorly matched ,HPV, types in cervical scrapes and most likely in the enlargement of the spectrum of HPVs detectable by this ...